The sensitivity to ozone of ten Bangladeshi wheat cultivars was tested by exposing plants to eight ozone exposure regimes (50, 60, 80, 100, 120, 135, 150, and 200 ppb for 14, 11, 8, 6, 5, 4, 3, and 1 days, respectively, for 8 h/day) in controlled environment chambers. Visible leaf injury, dry weight, chlorophyll, carotenoid content, leaf greenness (SPAD value), quantum yield of photosynthesis, and stomatal resistance were measured to evaluate response. Shoot biomass, total chlorophyll, leaf greenness, and carotenoid content were reduced in ozone-exposed plants. Based on the results of principal component analysis (PCA)-biplot analysis, the order of sensitivity to ozone was: Akbar >> Sufi ≥ Bijoy ≥ Shatabdi > Bari-26 ≥ Gourab > Bari-25 ≥ Prodip ≥ Sourav >> Kanchan. The most important parameters to discriminate cultivars with respect to ozone sensitivity were visible injury and chlorophyll b/a ratio, whereas quantum yield of photosynthesis was less important. Differences in stomatal resistance were not a significant factor in ozone response. Regression of cultivars’ PCA scores against year of release revealed no trend, suggesting that ozone tolerance was not incorporated during cultivar breeding.